TESTING RESULTS ABOUT WATER TREATMENT EFFECT IN AQUACULTURE FIELD OF SOME BIOLOGICAL PRODUCTS: GEM, GEM-P, CTA-T, CTA-8T

(Based on testing report of Experimental Biology Department _ Aquaculture Research Institute II)

 

Aquaculture Research Institute II has performed the test about some products such as: GEM, GEM-P, CTA-T, CTA-8T supplied by Consulting Center For Biology & Environment CTA. The results are reported as following:

    1. Information of products:

Name of products: GEM, GEM-P, CTA-T, CTA-8T

Classification: environmental treatment products

Manufacturer: Consulting Center For Biology & Environment CTA

Address: 66 Nguyen Van Troi, Phu Nhuan District, Ho Chi Minh city.

2. Testing method:
   1. Testing arrangement

Address: Experimental Aquaculture Area, 658 Kha Van Can, Linh Dong precinct, Thu Duc district, Ho Chi Minh city.

Time: from 15/10/2003 to 28/11/2003

Study object: Green crayfish

Breeding tank: 09 Composite tanks, 06 cement tanks 2.5m³ and 12 canvas pockets 1 m³.

Density: 20 crayfish/tank

Size: 80-100 crayfish/kg

Testing concentration:

• CTA – T: spraying CTA – T on the surface of tank with 2-3 ml/m³ for 5-7 days and blending into food with rate of 2 -3 %  based on food weight.
• CTA – 8T: spraying CTA – 8T on the surface of tank with 2-3 ml/m³ for 5-7 days and blending into food with rate of 2 -3 % based on food weight.
• GEM-P: improving environment: 100g/3000m², 1-2 time/month.
• GEM: 1-2 ppm, spray with rate of 5-7 days/1 time.

Testing time: test before each stage and after using for 7 days periodically.

1. Monitoring parameters

Some water-physiochemical quotas (NH₄⁺/NH₃, NO₂, pH, COD, alkaline level, DO)

3. Results

Utilized concentration is 2 ppm, 4 ppm of CTA – T, CTA – 8T, GEM, and 0.03 ppm; 0.05 ppm of GEM – P on composite and cement tanks before each stage and after 7 day- treatment. We have tested periodically water-physiochemical quotas at experimental plots and control-plots. Some quotas pH, alkaline level, DO are stable with testing time.

During experiment, we tested, evaluated and determined some elements NH₄⁺/NH₃, NO₂ and COD with the concentration of CTA – T, CTA – 8T is 2 ppm, 4 ppm; GEM is 0.03 ppm and GEM-P is 0.05 ppm compared with control plots.

The results are described as following:

• CTA-T product

 

Table II.4  The testing results of parameters after using CTA-T

 

 

Testing results of parameters NH₄⁺, NO₂-N, pH, alkaline level, DO after using CTA-T at concentration of 2ppm and 4ppm and control-sample are showed in image II.31

 

 

 

Note: Chỉ tiêu: Quotas Nồng độ: Concentration Đối chứng: Control Ngày: Day

Image II.31

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

• CTA-8T product

 

Table II.5 The testing results of parameters after using CTA-8T

 

 

Testing results of parameters NH₄⁺, NO₂-N, pH, alkaline level, DO after using CTA-T at concentration of 2ppm and 4ppm and control-sample are showed in image II.32

 

 

Image II.32

 

 

 

 

 

 

 

 

 

• GEM product

 

Table II.6 The results of parameters after using GEM-P

 

 

Testing results of parameters NH₄⁺, NO₂-N, pH, alkaline level, DO after using GEM-P at concentration of 2ppm and 4ppm and control-sample are showed in image II.33

 

 

Image II.33

• GEM product

Table II.7 The testing results of parameters after using GEM

 

 

Testing results of parameters NH₄⁺, NO₂-N, pH, alkaline level, DO after using GEM-P at concentration of 2ppm and 4ppm and control sample are showed in image II.34

 

Image II.34

 

According to above results, we showed that at the concentration of CTA – T, CTA – 8T, GEM is 2 ppm; GEM-P is 0.03 ppm, it isn’t significantly  different (P>0.05) between environmental elements compared with control-plot.

 

 At the experimental plots with concentration of CTA – T, CTA – 8T, GEM is 4 ppm and GEM-P 0.05 ppm and the same initial conditions,  it is significantly different (P<0.05) between environmental elements and suitable with breeding conditions, lower than control-plots.

 

4. Conclusion

CTA – T, CTA – 8T, GEM are used with concentration of 4 ppm and GEM –P 0.05 ppm in crayfish culture medium have ability of accumulation, organic mud decomposition, toxicant reduction, environmental stability (NH₄⁺/NH₃, NO₂ and COD are reduced).

According to testing evaluation report of Aquaculture Research Institute II - Ministry of Fisheries:

+ GEM and CTA – T are biological products containing useful microorganisms, can break up organic substances, waste things, excess food accumulated at pond floor, improve water quality.  

+ GEM-P and CTA – 8T are biological products containing useful microorganisms, natural Vitamins, micro minerals, stimulate alga development, decompose organic substances, reduce bad odor, stabilize color of water, improve water quality.

+ CTA – T, CTA – 8T, GEM, GEM-P  are biological products for water environmental treatment in aquaculture field, contain microorganisms and are used generally in aquaculture.    

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

   MINISTRY OF FISHERIES                         SOCIALIST REPUBLIC OF VIETNAM Aquaculture Research Institute II                         Independence – Freedom - Happiness

               ---o0o---                                                                     ---o0o---

                                                                    Ho Chi Minh city, February, 5th, 2004

 

EVALUATION REPORT

 

The board to evaluate testing results including:

• PhD. Nguyen Tuan, Scientific Board Chairman, Aquaculture Research Institute II.
• MA Le Duc Trung, Scientific Board Secretary, Aquaculture Research Institute II.
• Associate professor-PhD. Nguyen Tuong Anh, University of Natural Sciences,         Ho Chi Minh city.
• Engineer Bach Thi Quynh Mai, vice director of Processing Technology After Harvest And Aquatic Biology Center.

 

The board will evaluate testing results of some products such as GEM, CTA – T, GEM-P, CTA – 8T produced by Counting Center of Technology and Environment (CTA Center). After examining the report of testing results, the members of the Board give ideas and unify as following:

 

The contents of report includes: testing location, experimental arrangement, monitor water quality quotas.

 

GEM and CTA – T are biological products containing useful microorganisms, can break up organic substances, waste things, excess food accumulated at pond floor, improve water quality.  

 

GEM-P and CTA – 8T are biological products containing useful microorganisms, natural Vitamins, micro minerals, stimulate alga development, decompose organic substances, reduce bad odor, stabilize color of water, improve water quality.

 

CTA – T, CTA – 8T, GEM, GEM-P  are biological products for water environmental treatment in aquaculture field, contain microorganisms and are used generally in aquaculture.   

 

    Confirmed by                                           Rep. Evaluating Board

Aquaculture Research Institute II

Cao Thanh Hung                                         Ph.D. Nguyen Tuan

 

 

 

AQUACULTURE RESEARCH                       SOCIALIST REPUBLIC OF VIETNAM

   INSTITUTE II                                        Independence – Freedom - Happiness Experimental Biology Department                                               ---o0o---

                 ---o0o---                                                    

                                                         Ho Chi Minh city, January, 13th, 2004

 

 

TESTING REPORT

Products CTA – T, CTA – 8T, GEM, GEM-P

 

 

Aquaculture Research Institute II has performed some testes about products: CTA – T, CTA – 8T, GEM, GEM-P supplied by Consulting Center of Technology and Environment. Testing results are presented as following:

 

1. Information of products:

Name of product: GEM, GEM-P, CTA-T, CTA-8T

Classification: environmental treatment products

Manufacturer: Consulting Center For Biology & Environment CTA

Address: 66 Nguyen Van Troi, Phu Nhuan District, Ho Chi Minh city.

Components:

• CTA – T: Lactic bacteria ≥ 10⁹ CFU/ml, Autotropic bacteria ≥ 10⁹ CFU/ml, Aspergillus Oryzae ≥ 10³ CFU/ml and Saccharomyces cerevisiae ≥ 10⁹ CFU/ml.
• CTA – 8T: Lactic bacteria ≥ 10⁹ CFU/ml, Autotropic bacteria ≥ 10⁹ CFU/ml, Aspergillus Oryzae ≥ 10³ CFU/ml and Saccharomyces cerevisiae ≥ 10⁹ CFU/ml, natural vitamins E, C and natural antibiotics.
• GEM: Lactic bacteria ≥ 10⁹ CFU/ml, Autotropic bacteria ≥ 10⁹ CFU/ml, Aspergillus Oryzae ≥ 10³ CFU/ml and Saccharomyces cerevisiae ≥ 10⁹ CFU/ml.
• GEM-P: Lactic bacteria ≥ 10⁹ CFU/ml, Autotropic bacteria ≥ 10⁹ CFU/ml, Aspergillus Oryzae ≥ 10³ CFU/ml and Saccharomyces cerevisiae ≥ 10⁹ CFU/ml, SiO₂ = 42-45%, Al2O3 = 17-22%, MgO @ 1%, additives.

Use:

• CTA – T: Eliminate bad odor, reduce mud accumulated at pond floor, toxic gas NH₃-N, NO₂, stabilize water color, increase digestive ability.
• CTA – 8T: Inhibit the bacteria causing diseases (black gill, red body, worn out antenna), increase disease prevention ability of shrimp and reduce toxic gas NH₃-N, NO₂-N.
• GEM-P: Decrease time to create color for water, toxic gas NH₃-N, NO₂-N, reject bad odor, stabilize color of water.
• GEM: Reduce bad odor, mud, toxic gas accumulated at pond floor, control water quality.

Instruction for use:

• CTA – T: spray continuously CTA – T on the water surface with rate of 2-3 ml/m³ for 5-7 days in the early morning or dusk and blend into food with rate of 2 -3 % food weight, cause fermentation for 8-12 hours, feed everyday.
• CTA – 8T: spray continuously CTA – 8T on the water surface with 2-3 ml/m³ for 5-7 days in the early morning or dusk and blend into food with rate of 2 -3 % food weight, cause fermentation for 8-12 hours, feed with rate of 3-5days/time.
• GEM-P: Pond preparation: spread it regularly with rate of 200-500 g/2000m², keep the water level at 20 cm for 5-7 days; and improve environment: 100g/3000m², 1-2 times/month.
• GEM: Dissolve 1 liter of molasses in 20 liters of clear water, after that, put 1 liter of GEM into them, put the lid on the mixture-can and cause fermentation for 5-10 days at 27-30^oC (keep away from the sunlight). Then, open the lid of can, if the mixture has the faint sour aroma we can spray regularly on pond.

2. Testing method

1.  Testing arrangement

Location: Experimental Aquaculture Area.

Address: 658 Kha Van Can, Linh Dong precinct, Thu Duc district, Ho Chi Minh city.

Time: from 15/10/2003 to 28/11/2003

Study object: Green crayfish

Breeding tank: 09 Composite tanks, 06 cement tanks 2.5m³ and 12 canvas pockets 1 m³.

Density: 20 crayfish/tank

Size: 80-100 crayfish/kg

Testing concentration:

• CTA – T: spray CTA – T on the surface of tank with rate of 2-3 ml/m³ for 5-7 days and blend into food with rate of 2 -3 % food weight.
• CTA – 8T: spray CTA – 8T on the surface of tank with 2-3 ml/m³ for 5-7 days and blend into food with rate of 2 -3 % food weight.
• GEM-P: improve environment: 100g/3000m², 1-2 times/month.
• GEM: 1-2 ppm, 5-7 days/1 time.

Testing time: test before each stage and after using for 7 days periodically.

1.  Monitoring parameters

Some liquid and physiochemical quotas (NH₄⁺/NH³, NO², pH, COD, alkaline level, DO)

 

 

3. Results

Table 1. The testing results of parameters after using CTA-T

 

 

Table 2. The testing results of parameters after using CTA-8T

 

 

 

 

 

 

 

Table 3. The testing results of parameters after using GEM-P

 

 

Table 4. The testing results of parameters after using GEM

 

 

Utilized concentration is 2 ppm, 4 ppm of CTA – T, CTA – 8T, GEM, and 0.03 ppm; 0.05 ppm of GEM – P on composite and cement tanks before each stage and after 7day treatment. We have tested periodically water-physiochemical quotas at experimental plots and control plots. Some quotas pH, alkaline level, DO are stable with testing time.

After experiment, we tested, evaluated and determined some elements NH₄⁺/NH₃, NO₂ and COD with the concentration of CTA – T, CTA – 8T is 2 ppm, 4 ppm; GEM is 0.03 ppm and GEM-P is 0.05 ppm compared with control-plots. We showed that at the concentration of CTA – T, CTA – 8T, GEM is 2 ppm; GEM-P is 0.03 ppm, it isn’t significantly  different (P>0.05) between environmental elements compared with control-plot.

 

 At the experimental plots with concentration of CTA – T, CTA – 8T, GEM is 4 ppm and GEM-P 0.05 ppm and the same initial conditions,  it is significantly  different (P<0.05) between environmental elements and suitable with breeding conditions, lower than control-plots.

4. Conclusion:

CTA – T, CTA – 8T, GEM are used with concentration of 4 ppm and GEM –P 0.05 ppm in crayfish culture medium have ability of accumulation, organic mud decomposition, toxicant reduction, environmental stability (NH₄⁺/NH₃, NO₂ and COD are reduced).